Journal: Cell reports

Article Title: PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer

doi: 10.1016/j.celrep.2020.108253

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-CD34 conjugated to 148Nd (clone 581) , Fluidigm , Cat# 3148001B; RRID:AB_2810243.

Techniques: Recombinant, Multiplex Assay, SYBR Green Assay, In Situ, shRNA, Software

Journal: Cell Reports

Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants

doi: 10.1016/j.celrep.2022.111126

Figure Lengend Snippet:

Article Snippet: Anti-CD34 BB700 , BD Biosciences , 742246 (clone 563).

Techniques: Recombinant, Staining, Blocking Assay, Sequencing, Diagnostic Assay, Software, Digital PCR

Journal: Journal of Cellular and Molecular Medicine

Article Title: Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis

doi: 10.1111/jcmm.12548

Figure Lengend Snippet: TCs immunodiagnostics by double-labelled immunofluorescence. Dotted arrows indicated CD34-positive vascular endothelial cells. Negative c-kit staining was not shown here; scale bar = 20 μm. (A) CD34 (red) in moniliform cells overlying vimentin (green) cells with DAPI counterstaining (blue) in sham control (solid arrows), indicated the existence of perivascular TCs with special immunophenotype of CD34/vimentin double-positive. (B and C) CD34/vimentin double-positive cells with specific TCs morphology and well-defined nuclei was significantly less densely stained, reduced, sparse or completely absent (solid arrows) in AS-affected oviduct tissues. A statistically significant decrease in the mean number of TCs occurred ( P = 0.000). * P < 0.05 versus sham control; error bars = SD.

Article Snippet: Then FITC-goat antimouse IgG for vimentin (1:50; cat no. BA1101), CY3-goat anti-rabbit IgG for CD34 (1:50; cat no. BA1032), TRITC-goat anti-rabbit IgG for c-kit (1:50; cat no. BA1090) were added (all from Boster).

Techniques: Immunofluorescence, Staining, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis

doi: 10.1111/jcmm.12548

Figure Lengend Snippet: TCs and Tps damage in AS-affected oviduct tissues, accompanied by excessive amount of collagen fibres (Coll) and tissue fibrosis. (A) Intercellular connection between damaged TCs and activated mononuclear cells (MC). (a) Degenerated Tps established closed contact to activated MC which contained dense secretory granules, together with granulocyte infiltration, mainly eosinophils (Eo) and neutrophils (PMN). b higher magnification of the boxed area; (b) synapse (black arrows) between activated MC and degenerated Tps, which contained lots of swollen mitochondria (m) and vacuoles (white arrows), thus indicating degeneration, functional insufficiency of TCs and involvement of TCs in local immunoregulation. (B) Degeneration, discontinue or dissolution of TCs and Tps (black arrows), with cytoplasmic vacuolization (white arrows), accompanied by nearly normal scattered putative stem cells (SCs). Intercellular contacts between TCs and SCs was getting wider or disappeared (black asterisks). (C) Disrupted TC-SC niches which composed of a group of damaged Tps and putative SCs in myosalpinx, with heterocellular contacts getting wider or disappeared between Tps and SCs (black asterisks), Tps and activated Eosinophils (Eo) (white asterisk) with dense secretory granules respectively. Degeneration, discontinue or dissolution of TCs and Tps (black arrows), with swollen mitochondria (m), cytoplasmic vacuolization (white arrows) in Tps, swollen and dissolution of SMCs can be observed. (D) Severely damaged perivascular TCs and Tps, with swollen mitochondria (m), rough endoplasmic reticulum (rER) dilatation and cytoplasmic vacuolization (white arrows), together with damaged endothelial cell (E) and pericytes (P).

Article Snippet: Then FITC-goat antimouse IgG for vimentin (1:50; cat no. BA1101), CY3-goat anti-rabbit IgG for CD34 (1:50; cat no. BA1032), TRITC-goat anti-rabbit IgG for c-kit (1:50; cat no. BA1090) were added (all from Boster).

Techniques: Functional Assay, Dissolution

Journal: PLoS ONE

Article Title: In Vivo Serial MR Imaging of Magnetically Labeled Endothelial Progenitor Cells Homing to the Endothelium Injured Artery in Mice

doi: 10.1371/journal.pone.0020790

Figure Lengend Snippet: 7 days after in vitro differentiation, spleen-derived EPCs showed expression of CD31 (B), CD34 (C) and vWF (D) with the cytoplasm stained in brown. Photo A is the control. Hematoxylin-stained nuclei were shown in blue. (Magnification ×400).

Article Snippet: After 7 days in culture, spleen-derived EPCs in 4 wells of a 24-well culture plate were extensively washed, and fixed in ice-cold 4% paraformaldehyde for 20 min. Immunocytochemistry was performed using antibodies against CD31, CD34 (Wuhan Boster Biological Technology, LTD. Wuhan, China) and von Willebrand factor (vWF) ( Zhongshan Goldenbridge Biotechnology Co., LTD. Beijing, China) according to standard protocols.

Techniques: In Vitro, Derivative Assay, Expressing, Staining

Journal: Scientific Reports

Article Title: New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

doi: 10.1038/s41598-021-89616-3

Figure Lengend Snippet: Immunohistochemical staining images showing CD34 expression in ( A ) normal mouse brain tissue and ( B – F ) mouse brain tissue on days 3, 7, 15, 21 and 30 days, following the pTBI. ( A ) CD34 was not expressed in normal mouse brain tissue. CD34-positive cells and blood vessels (brown; black arrows) became visible at the injury site (red arrow) on days ( B ) 3 and ( C ) 7. ( D ) After 15 days, CD34 expression at the injury site (red arrow) decreased. ( E ) CD34 expression at the injury site could not be detected on day 21. Instead, black hemosiderin particles (black arrows) were observed at the injury site. ( F ) Some black hemosiderin particles remained visible at the injury site on day 30. ( G ) Immunohistochemical results of CD34 were analyzed by Image-Pro Plus 6.0 software. The average density (IOD) of CD34-positive cells in normal mouse brain tissue and in mouse brain tissue on days 3, 7, 15, 21 and 30 days, following pTBI. The n = 10/group was used for calculating the average IOD. Comparisons between groups were performed using the Kruskal–Wallis H test followed by Bonferroni post-hoc analysis.

Article Snippet: The sections were first incubated with rabbit anti-mouse PROX1 antibody (1:100; Boster Biological Technology Co., Wuhan, China), rabbit anti-mouse CD34 antibody (1:100; BosterBiological Technology) or goat anti-mouse LYVE-1 antibody (1:100; R&D Systems, Shanghai, China) at 4 °C for 24 h. Next, the sections were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG or rabbit anti-goat IgG secondary antibodies.

Techniques: Immunohistochemical staining, Staining, Expressing, Software

Journal: Scientific Reports

Article Title: New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

doi: 10.1038/s41598-021-89616-3

Figure Lengend Snippet: Double immunofluorescence staining images showing CD34/LYVE-1 expression in the brain tissue 3 days after pTBI. Expression of ( A ) LYVE-1 (green); ( B ) CD34 (red); and ( C ) merge (yellow).

Article Snippet: The sections were first incubated with rabbit anti-mouse PROX1 antibody (1:100; Boster Biological Technology Co., Wuhan, China), rabbit anti-mouse CD34 antibody (1:100; BosterBiological Technology) or goat anti-mouse LYVE-1 antibody (1:100; R&D Systems, Shanghai, China) at 4 °C for 24 h. Next, the sections were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG or rabbit anti-goat IgG secondary antibodies.

Techniques: Double Immunofluorescence Staining, Expressing